Alper Ciftci
Ondokuz Mayis University, Turkey
Title: Investigation of probiotic properties of chicken originated Enterococcus faecium and Lactobacillus species
Biography
Biography: Alper Ciftci
Abstract
Statement of the Problem: In this study, the isolation of Lactobacillus spp. and Enterococcus faecium and investigation of usage potential of isolated bacteria as probiotic were aimed.
Methodology & Theoretical Orientation: In a study, 50 chicken intestines were investigated which were taken from a commercial chicken slaughter house in Samsun region. The samples were taken from intestine mucosa and inoculated to the selective mediums for the isolation of Lactobacillus spp. and E. faecium. Suspected colonies were identified by PCR. The isolated bacteria were investigated for bile (0.5-1%) and pH (3-5) resistance by bile and pH tolerance tests, respectively. The hydrophobicity’s of the isolates were tested by using 0.03% Congo Red Agar. The antibiotic resistances of the isolates were determined by Agar Gel Disc Diffusion Test with using 9 antibiotic discs. The Radial Diffusion Method was used for determining the antagonistic effects of the isolates against Escherichia coli.
Findings: Twenty E. faecium, 21 Lactobacillus spp. (9 of them were L. acidophilus) isolated from samples. All the isolates were found as resistant to tested bile and pH conditions. All the isolates were hydrophobic, but none of them had an antagonistic effect against Escherichia coli. Eight of E. faecium isolates were found as resistant to 8 antibiotics. One Lactobacillus spp. (other than L. acidophilus) and 1 L. acidophilus isolates were resistant to 5 and 7 antibiotics, respectively. These isolates were evaluated as multi-antibiotic resistant strains.
Conclusion & Significance: In conclusion, we evaluated that all the isolates were hydrophobic, resistant to bile and low pH conditions; but none of them had an antagonistic effect against Escherichia coli in tested conditions. These results indicated that the multi-antibiotic resistant strains of E. faecium, L. acidophilus and Lactobacillus spp. isolates had a potential of using as a probiotic and further in vivo studies had to be essential for these strains.