7^th International Veterinary Congress

Biography

Biography: Belgin Sırıken

Abstract

Statement of the Problem: The objective of this study to find the prevalence and antimicrobial resistance of L. monocytogenes from a total of 116 chicken meat samples including 50 carcasses and 66 meat parts marketed in Turkey.

Methodology & Theoretical Orientation: In the study, immunomagnetic separation (IMS) based cultivation technique, to detect the hlyA gene for the verification of the isolates by PCR, and to identify the genoserotypes of the L. monocytogenes isolates by multiplex PCR assay. L. monocytogenes isolates were also tested for their susceptibility to eight antibiotic (gentamicin, vancomycin, chloramphenicol, streptomycin, tetracycline, ampicillin, penicillin G and erythromycin) agents using the disk diffusion method.

Findings: 51 L. monocytogenes colonies were isolated from 34 (29.3%) chicken meat samples (11 [22.0%] carcasses and 23 [34.8%] pieces of meat) by IMS based cultivation technique and confirmed by PCR. According to the multiplex PCR results, all the 51 isolates were identified as genoserotype IIa (1/2a or 3a), 14 isolates (27.45%) were susceptible to all eight antimicrobial drugs tested, and the remaining 37 isolates (72.54%) were resistant to gentamicin (one isolate 1.96), vancomycin (four isolates, 7.84%), penicillin G (six isolates, 1.76%), streptomycin (nine isolates, 17.64%; resistant or intermediate), tetracycline (seven isolates, 13.72%) and ampicillin (six isolates, 11.76%).

Conclusion & Significance: In conclusion, a relatively high prevalence of L. monocytogenes was noted and the isolates were characterized sporadic but sometimes epidemic serotypes. IIa in contrast to the most important epidemiological serotypes I. This study results also showed that antimicrobial resistance is not frequent in the isolates. However, we did not detect number of L. monocytogenes in analyzed samples. The presence of L. monocytogenes in chicken meat is concerning public health risk.