Surong Hasi
Inner Mongolia Agricultural University/ Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, China
Title: Study on in vivo activities of CYP3A enzyme in Bactrian camel with specific probe drug
Biography
Biography: Surong Hasi
Abstract
The aim of the research is to study the in vivo activities of Bactrian camels' CYP3A enzymes by investigating the pharmacokinetic characteristics of CYP3A specific probe drug Midazolam in Bactrian camel, and the effect of Itraconazole on the pharmacokinetic behavior of Midazolam was studied simultaneously. Firstly, five healthy adult Bactrian camels were intramuscularly injected with the single dose of 0.1 mg/kg Midazolam, and then blood samples were collected from the jugular vein at different time following the administration. Secondly, after 7 days drug clearance period, these five experimental camels were injected intramuscularly with the single dose of 0.1 mg/kg Itraconazole for 4 consecutive days, and following 2 h of last injection, Bactrian camels were administered intramuscularly with the single dose of 0.1 mg/kg Midazolam again. Blood samples were collected by same route and same intervals as previous, and the plasma was separated by centrifugation. The plasma concentration of Midazolam was determined high performance liquid chromatography-UV detection, and the pharmacokinetic parameters of Midazolam were analyzed by WinNonLin 7.0 with non-compartmental model. The pharmacokinetic parameters of Midazolam in group probe drug only and in group enzyme inhibitor plus probe drug were as follows: the T1/2 was 2.5±0.073 h and 3.674±0.29 h, Tmax was 0.85±0.09 h and 0.54±0.06 h, Cmax was 0.62±0.12 µg/mL and 0.80±0.06 µg/mL, AUC0-t was 1.47±0.35 h·µg/mL and 2.15±0.15 h·µg/mL, Vd was 259.17±41.29 mL/kg and 152.09±22.49 mL/kg, CL was 53.46±14.25 mL/h/kg and 34.3±5.13 mL/h/kg, and MRT was 3.71±0.16 h and 4.60±0.52 h, respectively. Therefore, all the T1/2, Tmax, Cmax and MRT of Midazolam in Bactrian camels were relatively low which indicated that Bactrian camels' CYP3A enzyme possess high activity on metabolism of Midazolam. Furthermore, the CYP3A enzyme was significantly inhibited by Itraconazole which can increase the T1/2, Cmax, AUC and MRT, and can reduce the Tmax of Midazolam in Bactrian camel